June 30, 2016
11 am to 12 pm EDT

Sponsored by

Webinar Description:

Comparative Evaluation of the Simple Plex Immunoassay with other Multiplexing Technologies in a Biological Context. Quantitative measurement of proteins is essential for evaluation of biomarkers and biological processes, yet this common technique is impractical to evaluate multiple proteins, owing to cross-reactivity, poor reproducibility and lack of sensitivity. Simple Plex technology offers a new alternative to multiplexing through its spatial separation of target analytes into distinct microfluidic channels, and the ability to automate the analysis within a low volume cartridge. In this webinar, Paulomi Aldo will present results from her recent study to characterize and validate the Simple Plex platform for protein biomarker detection in human and complex samples. Comparative methods such as Luminex showed trends similar to Simple Plex, across a wide range of human and rodent sample types. Overall, Simple Plex demonstrated significant benefit over common multiplexed technologies in terms of low sample volume requirements, greater sensitivity and dynamic range, and time savings.

Learning Objectives:

• Comparison and data analysis of different multiplexed immunoassay platforms for detection of biomarkers.
• Advantages of the Simple Plex platform versus other multiplexing technologies in the multi-user environment of a core facility.
• How Simple Plex eliminates the challenges of cross-reactivity, assay optimization, and poor reproducibility inherent in multiplexed immunoassays.

Speaker:

Paulomi Aldo

Research Associate

Yale University

Paulomi Aldo received her undergraduate training in Biology and Psychology from Salve Regina University and her Masters of Science degree in Molecular and Cellular Biology at the University of New Haven. She is currently a Research Associate at Yale University School of Medicine investigating the role of viruses and the immune system in pregnancy complications.

Introducer:

Gregory Marusov, PSM

Research Scientist, Assay Development

ProteinSimple

Gregory Marusov received his undergraduate degree is in Molecular and Cell Biology from the University of Connecticut, and obtained a Professional Science Master’s (PSM) degree in Applied Genomics from U of Connecticut. Prior to joining ProteinSimple, he developed technologies at HTS Biosystems for multiplexed surface plasmon resonance imaging of protein arrays. He is now a research scientist responsible for new assay development within the Simple Plex product line.