Webinar Description:

FFPE tissue and samples with a low abundance of high-quality nucleic acid represent a bottleneck in sample preparation workflows. Researchers and clinicians working with these samples often resort to labor-intensive methods in order to isolate sufficient material for downstream analyses. However, such methods are not always successful in increasing yields and can introduce bias which further impacts the quality of downstream analysis.

Purigen Biosystems has recently launched the revolutionary Ionic® Purification System to help scientists overcome the sample preparation bottleneck with a simple, automated approach to nucleic acid purification using isotachophoresis. Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip. An electric field is then applied to the chip and the nucleic acid is isolated in its natural, native form. The nucleic acid is not denatured or dehydrated, and there’s no binding to, or stripping from, fixed surfaces.The result is a higher yield of pure nucleic acid that is less fragmented and free from bead or wash buffer contamination.

In this webinar, we will discuss downstream molecular analyses of RNA/DNA purified from FFPE samples, fresh-frozen tissue, and cultured cells using the Purigen Ionic® Purification System. The results from quality assessments and downstream NGS will be emphasized.

Learning Objectives:

• A new innovative approach to automated nucleic acid purification
• How to extract more DNA and RNA from challenging FFPE samples with more reliability and less effort by comparison to conventional extraction methods

Speaker:

Lewis Marshall
Director of Microfluidics Engineering
Purigen Biosystems

Lewis Marshall is a scientific researcher with a focus on electrophoresis and Isotachophoresis. He received his Ph.D. in Chemical Engineering from Stanford University in 2013, prior to joining Purigen Biosystems. As the Director of fluidic engineering at Purigen Biosystems, Lewis has led the development of automated systems and fluidic devices to purify nucleic acids. He currently leads a team responsible for the development of products to purify nucleic acid from FFPE tissue.