July 15, 2015
11 am to 12 pm EDT

Sponsored by

Webinar Description:

We will describe the use of single molecule arrays (Simoa) for the multiplex detection of proteins at very low concentrations. We will provide an introduction to the Simoa technology--which is capable of provided a 1000-fold improvement in sensitivity to proteins over conventional assays--and describe how it can be used to detect multiple proteins at the single molecule level. The Simoa HD-1 Analyzer that allows multiplex Simoa assays to be fully-automated from samples-in to results-out will also be described. Simoa users will describe how they have used this technology, including development of a 6-plex for inflammatory cytokines and application of the multiplex Simoa homebrew kit.

Learning Objectives:

• Understanding of basics of the Simoa technology and Simoa HD-1 Analyzer
• Understanding of multiplex, single molecule protein measurements
• Background on the use of multiplex Simoa homebrew kit
• Application of Simoa to the multiplex measurement of inflammatory cytokines

Speakers:

David Duffy, Ph.D.

Vice President, Research and Chief Technology Officer

Quanterix

Dr. Duffy joined Quanterix in 2007 and leads the team of scientists developing its single molecule detection technology. Dr. Duffy was previously at Surface Logix, where he was the Director of Pharmacomer Technology. There he oversaw the development of a novel chemical technology for optimizing the PK/PD of small molecules that resulted in two drug candidates currently in Phase II clinical trials. Prior to that, Dr. Duffy was at Gamera Biosciences where he was a co-inventor of a centrifugal microfluidic device called the LabCD that was acquired and commercialized by Tecan. Dr. Duffy is an inventor on 12 U.S. patents and has more than 20 publications in the fields of surface chemistry, microfluidics, and bioanalysis. Dr. Duffy was a postdoctoral research fellow in the Department of Chemistry and Chemical Biology at Harvard University. He was the first Sir Alan Wilson Research Fellow of Emmanuel College, University of Cambridge. Dr. Duffy obtained his Ph.D. in physical chemistry from the Department of Chemistry at the University of Cambridge, and his B.A. and M.A. degrees in Natural Sciences from Selwyn College, University of Cambridge.

Andrew Rivnak

Scientist

Quanterix

Andrew joined Quanterix over 7 years ago after earning his B.A. in Biology from Tufts University and working at the Walt Lab at Tufts under Quanterix’ scientific founder Dr. David Walt. He has been instrumental in spearheading the multiplex model assay development project and working with the scientific and software teams at Quanterix to develop and improve the Simoa HD-1 instrument. Andrew’s recent work was published in the Journal of Immunological Methods in May 2015.

Jérémie Decalf, Ph.D.

Pasteur Institute

Danlu Wu, Ph.D.

Tufts University

Danlu Wu is currently a postdoc scholar in Dr. David Walt’s group at Tufts University, focusing on immunoassay development for early stage infectious disease diagnostics using Simoa technology. Before that, she spent five and half year in graduate school at University of Florida. She received a Master Degree in Materials Science and a Ph.D. in Analytical Chemistry with research interesting in materials characterization and protein biosensor development. She graduated from University of Science and Technology of China with a Bachelor of Science Degree in Polymer Chemistry before coming to United States.