May 21, 2015
11 am to 12 pm EDT

Sponsored by

Webinar Description:

Biophysical techniques based on static and dynamic light scattering address many of the key analytical challenges in biotherapeutic R&D, from early candidate selection through scale-up, formulation, characterization and comparability studies. This seminar will review light scattering technology and instrumentation, then present select examples illustrating how complete light scattering solutions facilitate rapid and effective development of biologics including mAbs, ADCs, PEGylated and other proteins as well as viruses, VLPs and nanoparticle drug-delivery vehicles.

Learning Objectives:

• How size-exclusion chromatography coupled to multi-angle light scattering (SEC-MALS) is used to determination of molar mass, size and composition for biopolymers and protein conjugates
• How field-flow fractionation coupled to MALS (FFF-MALS) is used to perform high-resolution characterization of size, conformation and composition of virus, VLP, liposome, and emulsion nanoparticles as well as sub-micron protein aggregates, by FFF-MALS
• How high-throughput dynamic light scattering (HT-DLS) carried out in microwell plates is utilized in biotherapeutic formulation and stability studies
• How electrophoretic light scattering (ELS or PALS) can determine molecular charge or nanoparticle zeta potential in standard formulation conditions to enhance formulation studies of biologics and vaccines
• How label-free, immobilization-free analysis of antigen-antibody and more complex biomolecular interactions is readily accomplished by composition-gradient MALS (CG-MALS)

Who should attend:

• R&D managers who encounter common challenges associated with biophysical characterization.
• Researchers interesting in learning about new analytical techniques applicable to biotherapeutic research and development.
• Staff scientists involved in the development of drug delivery.
• Project Supervisors needing more information about cutting-edge technology for protein, biopolymer and nanoparticle characterization.

Speaker:

Daniel Some, Ph.D.

Principal Scientist

Wyatt Technology Corp.

Daniel Some is Principal Scientist as well as the Director of Marketing at Wyatt Technology Corp. He has been with Wyatt for over ten years, first in R&D and then in the marketing department. Prior to joining the scientific instrumentation world his professional endeavors included the semiconductor and defense industries. Dr. Some completed his undergraduate degree in physics at the Technion Israel Institute of technology, his doctoral research in the Brown University physics department, and postdoctoral research at Los Alamos National Lab and the Weizmann Institute of Science